Serrano–Lowe oncogene-induced senescence experiment
The Serrano–Lowe oncogene-induced senescence experiment was a 1997 study showing that an activated oncogene pushes primary cells into a senescence-like growth arrest. Manuel Serrano, Scott Lowe, and colleagues expressed oncogenic ras in primary human and rodent fibroblasts and observed a permanent G1 arrest accompanied by accumulation of p53 and p16^INK4a. The arrest depended on those two tumor-suppressor pathways. The result identified senescence as an early barrier to cancer and connected the Hayflick–Moorhead phenotype to oncogenic stress.1
Background
An active ras gene transforms immortal rodent cell lines to a tumorigenic state, while primary cells resist ras alone. Land, Parada, and Weinberg had shown in 1983 that primary embryo fibroblasts become tumorigenic only when ras arrives with a
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